Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment

Authors

  • Mohammad Nabi Sarboluki Institute of Biochemistry and Biophysics, Tehran University, P.O. Box 13145-1384, Tehran, I.R. IRAN
  • Roya Mahinpour Department of Chemistry, Kashan University, Zip code 87304, Kashan, I.R. IRAN
Abstract:

Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yield (~21%) was obtained under the following conditions: enzyme/substrate ratio 0.1; amplitude 18mm (peak to peak), and exposure time 30 minutes. This yield is 30X that of the starting raw material, 10X that of the untreated rice-straw cellulose and 12-13X that of the value reported by others regarding pretreatment of wheat-straw cellulose by phenol. Changing the enzyme/substrate ratio to 0.4 raises glucose yield to 42% under the same conditions. Removal of temperature control during pretreatment (50-80 °C) increases the yield up to ~30% without increasing the enzyme/substrate ratio.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

enhancing enzymatic hydrolysis of cellulose by ultrasonic pretreatment

slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. maximum glucose yield ...

full text

Effects of Ultrasonic and High-Pressure Homogenization Pretreatment on the Enzymatic Hydrolysis and Antioxidant Activity of Yeast Protein Hydrolysate

Protein hydrolysate is highly regarded as a source of naturally occurring antioxidant peptides. The purpose of this study was to investigate the effect of Ultrasonic (Frequency, 20 KHz; Amplitude, 50%; Time, 30 min) and high-pressure homogenization (Power, 1500 bar; Rated flow, 10 dm/h) pretreatmenton the enzymatic hydrolysis and antioxidant properties of yeast protein hydrolysate obtained from...

full text

Mitigation of cellulose recalcitrance to enzymatic hydrolysis by ionic liquid pretreatment.

Efficient hydrolysis of cellulose-to-glucose is critically important in producing fuels and chemicals from renewable feedstocks. Cellulose hydrolysis in aqueous media suffers from slow reaction rates because cellulose is a water-insoluble crystalline biopolymer. The high-crystallinity of cellulose fibrils renders the internal surface of cellulose inaccessible to the hydrolyzing enzymes (cellula...

full text

Pretreatment and Enzymatic Hydrolysis of Sorghum Bran1

Cereal Chem. 84(1):61–66 Sorghum bran has potential to serve as a low-cost feedstock for production of fuel ethanol. Sorghum bran from a decortication process (10%) was used for this study. The approximate chemical composition of sorghum bran was 30% starch, 18% hemicellulose, 11% cellulose, 11% protein, 10% crude fat, and 3% ash. The objective of this research was to evaluate the effectiveness...

full text

Accelerating enzymatic hydrolysis of chitin by microwave pretreatment.

Response surface analysis was used to determine optimum conditions [2% (w/v) chitin, 57.5 degrees C, 38 min] for microwave irradiation of chitin to improve its enzymatic hydrolysis. V(max)/K(m) of cabbage chitinase toward untreated and microwave-irradiated chitin was found to be 21.1 and 31.7 nmol h(-1) mg(-2) mL, respectively. Similar improvement was observed in the case of pectinase in its un...

full text

Effect of Cellulose Structure on Enzymatic Hydrolysis

Enzymatic hydrolysis of non-dried and dried cellulose samples having various particles size, degree of polymerization, porosity, crystalline polymorph, and content of non-crystalline domains has been studied. Regression analysis was carried out to determine contribution of various structural features of cellulose samples to their hydrolysability. It was found that particle size, degree of polym...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 17  issue 1

pages  8- 13

publication date 1998-06-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023